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Hepato-Renal Toxicity of Gongronema latifolium Extracts on Streptozocin Induced Diabetes in Rats

Received: 22 April 2016     Accepted: 7 May 2016     Published: 14 May 2016
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Abstract

This study evaluated the effect of methanolic, ethanolic, aqueous and crude extracts of Gongronema latifolium leaves on some nephrotoxic and hepatotoxic parameters in streptozocin induced diabetic rats. Thirty albino rats divided into six experimental groups were used. Two groups served as control were fed normal saline and glibenclamide. Four groups were fed methanolic, ethanolic, crude and aqueous extracts of Gongronema latifolium leaves respectively. Treatment lasted four 14 days after which the rats were sacrificed and blood collected for biochemical evaluation. The results showed that treatment with all the various extracts of Gongronema latifolium leave did not cause any significant increase in the nephrotoxic and hepatotoxic parameters. Treatment with methanolic extract caused a significant decrease in the serum glutamate pyruvate transaminase activity (P<0.001). The histological analysis revealed a recovery from inflammatory phase of the cells of the kidney and liver from the toxic effect of the streptozocin.

Published in American Journal of Health Research (Volume 4, Issue 3)
DOI 10.11648/j.ajhr.20160403.15
Page(s) 62-69
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Hepatoxicity, Nephrotoxicity, Gongronema latifolium, Diabetes, Plant Extracts, Phytochemicals

References
[1] Punitha R., Shirwaika A, Shirwaikar A (2005). Anti-diabetic activity of benzyl tetraisoquinoline alkaloid, berberine, in streptozocin-nicotinamide induced type 2 diabetic rats. Diabetologian Croat., 34 (4): 117-121.
[2] Eleyinmi A. F. Sporns P. and Bressler D. C., 2008. Nutritional composition of Gongronema latifolium and Vernonia amygdalina. Nutrition & Food Science, 38 (2), 99–109.
[3] Saidu A. N and Okorocha S. C (2013). Phytochemical Screening and Hypoglycemic Effect of Methanolic Extract of Gongronema latifolium leaf in alloxan induced diabetic rats. Journal of Emerging Trends in Engineering and Sciences, 4 (6), 855-858.
[4] Jafri M. A., Subhani M. J., K. Javed K. and Singh S. (1999). Hepatoprotective activity of leaves of Cassia occidentalis against paracetamol and ethyl alcohol intoxication in rats. Journal of Ethnopharmacology, 66: 355–61.
[5] Vashishtha V. M., John T. J. and Kumar A. (2009). Clinical and pathological features of acute toxicity due to Cassia occidentalisin vertebrates. Indian Journal of Medical Research, 130: 23–30.
[6] Balogun M. E., Besong E. E., Obimma J. N., Mbamalu O. S. and Djobissie S. F. A. (2016). Latifolium: A Phytochemical, Nutritional and Pharmacological Review, Journal of Physiology and Pharmacological Advances, 6 (1): 811-824.
[7] Effiong G. S., Udoh I. E., Mbagwu H. O. C., Ekpe I. P., Asuquo E. N., Atangwho I. J. and Ebong P. E (2012). Acute and chronic toxicity studies of the ethanol leaf extract of Gongronema latifolium. International Research Journal of Biochemistry and Bioinformatics, 2 (7), 155-161.
[8] Augustine O., Nonye C. N. and Nicholas T. K. D. (2012). Phytochemical characterization and comparative efficacies of crude extracts of carica papaya. International Journal of Drug Research and Technology, 2 (5), 399- 406.
[9] Etim O. E., Akpan, E. J. and Usoh, I. F., 2008. Hepatotoxicity of carbon tetrachloride: protective effect of Gongronema latifolium. Pakistan Journal of Pharmaceutical Sciences 21 (3): 269–274.
[10] Shi J., Asiaki K., Ikawa Y. and Wake K. (2003). Evidence of hepatocyte apoptosis in rat liver after the administration of carbontetrachloride. Journal of Medical Research, 4: 1-8.
[11] Chung H. S., Chong, L. C., Lee S. K., Shamon L. A., Breemen R. B. V., Mehta R. G., Farnsworth N. R., Pezzuto J. N. and Kinghorn A. D. (1999). Flavonoids constituents of chlorinzan diffused with potential cancer chemopreventive activity. Journal of Agriculture Food Chemistry, 47: 35-4.
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    Brown Holy, Tamunoemine Davies, Thompson N. Imomoemi. (2016). Hepato-Renal Toxicity of Gongronema latifolium Extracts on Streptozocin Induced Diabetes in Rats. American Journal of Health Research, 4(3), 62-69. https://doi.org/10.11648/j.ajhr.20160403.15

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    ACS Style

    Brown Holy; Tamunoemine Davies; Thompson N. Imomoemi. Hepato-Renal Toxicity of Gongronema latifolium Extracts on Streptozocin Induced Diabetes in Rats. Am. J. Health Res. 2016, 4(3), 62-69. doi: 10.11648/j.ajhr.20160403.15

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    AMA Style

    Brown Holy, Tamunoemine Davies, Thompson N. Imomoemi. Hepato-Renal Toxicity of Gongronema latifolium Extracts on Streptozocin Induced Diabetes in Rats. Am J Health Res. 2016;4(3):62-69. doi: 10.11648/j.ajhr.20160403.15

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  • @article{10.11648/j.ajhr.20160403.15,
      author = {Brown Holy and Tamunoemine Davies and Thompson N. Imomoemi},
      title = {Hepato-Renal Toxicity of Gongronema latifolium Extracts on Streptozocin Induced Diabetes in Rats},
      journal = {American Journal of Health Research},
      volume = {4},
      number = {3},
      pages = {62-69},
      doi = {10.11648/j.ajhr.20160403.15},
      url = {https://doi.org/10.11648/j.ajhr.20160403.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajhr.20160403.15},
      abstract = {This study evaluated the effect of methanolic, ethanolic, aqueous and crude extracts of Gongronema latifolium leaves on some nephrotoxic and hepatotoxic parameters in streptozocin induced diabetic rats. Thirty albino rats divided into six experimental groups were used. Two groups served as control were fed normal saline and glibenclamide. Four groups were fed methanolic, ethanolic, crude and aqueous extracts of Gongronema latifolium leaves respectively. Treatment lasted four 14 days after which the rats were sacrificed and blood collected for biochemical evaluation. The results showed that treatment with all the various extracts of Gongronema latifolium leave did not cause any significant increase in the nephrotoxic and hepatotoxic parameters. Treatment with methanolic extract caused a significant decrease in the serum glutamate pyruvate transaminase activity (P<0.001). The histological analysis revealed a recovery from inflammatory phase of the cells of the kidney and liver from the toxic effect of the streptozocin.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - Hepato-Renal Toxicity of Gongronema latifolium Extracts on Streptozocin Induced Diabetes in Rats
    AU  - Brown Holy
    AU  - Tamunoemine Davies
    AU  - Thompson N. Imomoemi
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    DO  - 10.11648/j.ajhr.20160403.15
    T2  - American Journal of Health Research
    JF  - American Journal of Health Research
    JO  - American Journal of Health Research
    SP  - 62
    EP  - 69
    PB  - Science Publishing Group
    SN  - 2330-8796
    UR  - https://doi.org/10.11648/j.ajhr.20160403.15
    AB  - This study evaluated the effect of methanolic, ethanolic, aqueous and crude extracts of Gongronema latifolium leaves on some nephrotoxic and hepatotoxic parameters in streptozocin induced diabetic rats. Thirty albino rats divided into six experimental groups were used. Two groups served as control were fed normal saline and glibenclamide. Four groups were fed methanolic, ethanolic, crude and aqueous extracts of Gongronema latifolium leaves respectively. Treatment lasted four 14 days after which the rats were sacrificed and blood collected for biochemical evaluation. The results showed that treatment with all the various extracts of Gongronema latifolium leave did not cause any significant increase in the nephrotoxic and hepatotoxic parameters. Treatment with methanolic extract caused a significant decrease in the serum glutamate pyruvate transaminase activity (P<0.001). The histological analysis revealed a recovery from inflammatory phase of the cells of the kidney and liver from the toxic effect of the streptozocin.
    VL  - 4
    IS  - 3
    ER  - 

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Author Information
  • Dept. of Medical Laboratory Science Rivers State University of Science and Technology, Npkolu, Port Harcourt, Nigeria

  • Dept. of Medical Laboratory Science Rivers State University of Science and Technology, Npkolu, Port Harcourt, Nigeria

  • Dept. of Medical Laboratory Science Rivers State University of Science and Technology, Npkolu, Port Harcourt, Nigeria

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